Cisplatin binding to holliday junction

WebK Komori, S Sakae, H Shinagawa, K Morikawa, Y Ishino, A Holliday junction resolvase from Pyrococcus furiosus: Functional similarity to Escherichia coli RuvC provides … WebMar 15, 1997 · The results confirm that RuvC protein binds Holliday junctions as a dimer. Influence of DNA sequence on incision Previously it was shown that Holliday junction cleavage by RuvC occurs at specific sequences, with the consensus defined as 5′- A / T TT ↓G / C -3′. Single base changes within this sequence severely reduce the efficiency of …

Nanopore Analysis as a Tool for Studying Rapid Holliday Junction ...

Webformation of Holliday junction 1. after strand invasion, the two DNA molecules are connected by crossing DNA strands to form the Holliday junction 2. junction can move along the DNA by repeated DNA melting and formation of base pairs 3. process is called branch migration resolution of Holliday junction WebJul 2, 2004 · The RuvC protein binds specifically to a Holliday junction and cleaves it in one of the two possible orientations [16], [17], [18]. No spontaneous or cisplatin-induced … dictionary asynchronous https://lemtko.com

Binding to four-way junction DNA: a common property of

WebAug 5, 2011 · Holliday junctions are formed during recombination, double-strand break repair, and fork reversal during replication. Resolving Holliday junctions is a critical process for maintaining genomic stability [ 25, 26 ]. These junctions are resolved by a class of structure-specific nucleases: the junction-resolving enzymes. Figure 1 WebFour-way DNA intermediates, called Holliday junctions (HJs), can form during meiotic and mitoticrecombination,andtheirremovaliscrucialforchromosomesegregation.Agroupof ubiquitous and highly specialized structure-selective endonucleases catalyze the cleavage of HJs into two disconnected DNA duplexes in a reaction called HJ resolution. WebTo expose two cut sites, the Holliday junction is rotated by 180° to form a sequare planar structure. Resolution of Holliday junction occurs by cutting the DNA strands at the site of cross and re-joining them. Resolution occurs in one of the two ways. This gives rise to two classes of DNA products. dictionary astonish

The RuvC protein dimer resolves Holliday junctions by a dual …

Category:Holliday junction binding by the BCDX2 complex. (A) The …

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Cisplatin binding to holliday junction

Structural Similarities between Escherichia coli Ruva Protein …

WebJul 5, 2024 · Holliday junctions (HJs) are an important class of nucleic acid structure utilized in DNA break repair processes. As such, these structures have great importance as therapeutic targets and for...

Cisplatin binding to holliday junction

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WebDNA polymerases fill in 3′ overhangs, whereby the D-loop becomes a Holliday junction. RAD52 functions to anneal the second end of DNA leading to a Holliday junction. BLM migrates the two ends of the Holliday junction in the direction of one another with some filling in by polymerases if necessary. WebThe RuvAB complex can carry out DNA helicase activity, which helps unwind the duplex DNA. The binding of the RuvC protein to the RuvAB complex is thought to cleave the …

WebAbstract. In the present paper, the interactions of the origin binding protein (OBP) of herpes simplex virus type 1 (HSV1) with synthetic four-way Holliday junctions (HJs) were … WebAug 27, 2024 · Brogden, A.L.; Hopcroft, N.H.; Searcey, M.; Cardin, C.J. Ligand bridging of the DNA Holliday junction: Molecular recognition of a stacked-X four-way junction by a …

WebSep 15, 2024 · Cisplatin (Cisplatin Injection) may treat, side effects, dosage, drug interactions, warnings, patient labeling, reviews, and related medications including drug … WebHolliday junctions (Holliday, 1964) are four-way junctions in DNA formed by the mutual exchange of strand connectivity, and thus connected by the covalent continuity of each strand of the junction ( Orr-Weaver, Szostak, & Rothstein, 1981; Potter & Dressler, 1976; Schwacha & Kleckner, 1995 ).

WebThe BCDX2 complex also bound to the synthetic Holliday junction, and its af®nity to the Holliday-junction DNA was signi®cantly higher than that of Rad51B (Fig. 4).

WebThe binding of Holliday junction DNA by endo-exonuclease. Purified endo-exonuclease (5 units) was incubated with 32P-labeled junction molecules, linear ss-DNA (146 nucleotides), and linear ds-DNA ... dictionary atlasWebPMID: 9535214 Abstract Proteins that can be shown to strongly bind in vitro to the four-way (Holliday) junction DNA include not only the obvious candidates such as enzymes involved in recombination, but also a remarkably diverse group of seemingly unrelated proteins. city code helWebCX3 (A) or BCDX2 (B) was incubated with Holliday junction templates, mounted onto carbon-coated copper grids, and rotary shadowcast with tungsten for visualization by EM. Images are shown in ... dictionary at leastWebBinding of RuvA to the Holliday Junction109 phosphate atoms across the minor groove in B-DNA. It is tempting to speculate that the arrangement of the two approximately 2-fold- related HhH... dictionary attack in cybersecurityWebNational Center for Biotechnology Information dictionary atonementWebway DNA structure, also called a Holliday junction, which is processed by the RuvABC proteins.2 The RuvA protein initially binds the Holliday junction, and then recruits RuvB protein rings to opposite sides of the junction.3,4 RuvB is a molecular motor that uses the energy derived from ATP binding and hydrolysis to drive unidirectional movement ... city code hhnWebFeb 1, 2003 · When the synthetic Holliday junction was present along with the half-cruciform and double-stranded oligonucleotides, the Rad51B protein only bound to the synthetic Holliday junction, which... dictionary a to z