Orfh79

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August undefined, 2024

Witryna30 mar 2024 · The chimeric orfH79 from CMS-HL is a variant of orf79 from CMS-BT that encodes proteins with a 5′ region similar to cox1 and a 3′ region of unknown origin [39,40]. For the rice RT98A CMS line, its orf113 has the same sequence as nad9 in the region from –151 to +11, while the rest is composed of unknown sequences [ 41 ]. Witryna2 wrz 2008 · Abstract. For years discovery and identification of the cytoplasmic male sterility (CMS) resource in wild rice is the most intriguing events in breeding field. …

Gametophytically alloplasmic CMS line of rice (Oryza sativa L.) with ...

Witryna7 lut 2024 · Results: The B-atp6-orfH79 and its variants (B-atp6-GSV) were detected in five among seventeen populations (i.e. HK, GZ, PS, TL and YJ) through PCR … Witryna7 lut 2024 · In this work, the B-atp6-orfH79, orfH79 and atp6 in 427 individuals of seventeen O. rufipogon populations in China were amplified using specific primers … pregnancy breakfast recipes indian

Western blot analysis of ORFH79 in whole E. coli extracts and ...

Witryna7 lis 2024 · To investigate the distribution of original CMS source, and explore the origin of gametophytic CMS gene, a total of 427 individuals with seventeen representative populations of O. rufipogon were collected in from Dongxiang of Jiangxi Province to Sanya of Hainan Province, China, for the PCR amplification of atp6, orfH79 and B-a … Witryna24 cze 2010 · The mitochondrial gene orfH79 is a candidate gene for causing the CMS trait in CMS-Honglian (CMS-HL) rice. However, whether the orfH79 expression can … WitrynaPreviously, orfH79 was cloned and identified as the CMS gene in Honglian rice, but how the ORFH79 protein causes pollen abortion is still unknown. Through bacterial two-hybrid library screening, P61, a subunit of the mitochondrial electron transport chain (ETC) complex III, was selected as a candidate that interacts with ORFH79. scotch map taste

Accelerated molecular breeding of novel cytoplasmic male sterility ...

WitrynaThe mitochondrial gene orfH79 is a candidate gene for causing the CMS trait in CMS-Honglian (CMS-HL) rice. However, whether the orfH79 expression can actually induce CMS in rice remains unclear.ResultsWestern blot analysis revealed that the ORFH79 protein is mainly present in mitochondria of CMS-HL rice and is absent in the fertile line. Witryna24 cze 2010 · The mitochondrial gene orfH79 is a candidate gene for causing the CMS trait in CMS-Honglian (CMS-HL) rice. However, whether the orfH79 expression can … pregnancy breast stretch marks picturesWitryna24 cze 2010 · The orfH79 gene and its variants (hereafter abbreviated as GSV for convenience) are adjacent to and co-transcribed with B-atp6 and it's a 52 bp downstream connected by an abundant variance 176 bp ... scotch margarita

"Witryna25 lut 2013 · The orfH79 gene fused with glutathione S-transferase (GST) protein and GST protein alone were expressed and purified by Escherichia coli BL21 with … " - Orfh79

Orfh79

Accelerated molecular breeding of novel cytoplasmic male sterility ...

Witryna25 lut 2013 · We propose that the CMS protein, ORFH79, can bind to complex III and decrease its enzyme activity through interaction with P61. This defect results in … Witryna2 sie 2024 · Based on the studies on HL-type hybrid rice, the orfH79 gene expression element was synthesized by Qingke Biotechnology Co., Ltd., containing the promoter PG47, the RF1b MSP sequence and the terminator IN2-1, and was introduced into pCAMBIA1300. Then, the construct pCAMBIA1300-orfH79 was transformed into …

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Witryna19 sty 2016 · If RFC3 directly binds CMS RNA, atp6-orfH79, translation of ORFH79 would be blocked by overexpression of RFC3 in sterile lines. However, overexpression of RFC3 did not rescue sterility, implying that RFC3 cannot bind CMS RNA, atp6-orfH79, and this is consistent with the role of GRP162 in the interaction between the complex … Witryna1 maj 2002 · In HL-CMS, atp6-orfH79 is the CMS-associated transcript. Although identity between orfH79 and orf79 sequences (from HL-CMS and BT-CMS, respectively) has a commonality of 98%, they show significant ...

WitrynaUmożliwiające „zapamiętanie” wybranych przez użytkownika ustawień tak, aby nie musiał dostosowywać ustawień strony do swoich preferencji za każdym razem, np. w … Witryna4 lut 2024 · Based on the alignment of amino acid sequences, four accessions with orfH79 and three accessions with orf290 were screened. Four novel CMS lines carrying orfH79 haplotypes and three novel CMS lines carrying orf290 haplotypes were then developed using multiple backcross generations with a maintainer line under MAS. …

WitrynaOne copy of atpt6 in sterile line was same to that in maintainer line; the other showed different flanking sequence from the 49th nucleotide downstream of the termination codon of atp6 gene. A new chimeric gene, orfH79, was found in the region. OrfH79 had homology to mitochondrial gene cox II and orf107, and was special to HL-sterile … Witryna7 maj 2013 · The restoration fertility complex (RFC) was previously identified in Honglian (HL)-cytoplasmic male sterility (CMS) rice (Oryza sativa), and glycine-rich protein 162 (GRP162) is responsible for binding to the CMS-associated transcript atp6-orfH79.Here, we engineered a recombinant GRP162 containing the mitochondrial transit peptide, …

WitrynaSzkoła Podstawowa nr 79 im. Łódzkich Olimpijczyków w Łodzi, Łódź. 1480 osób lubi to · 236 osób mówi o tym · 293 użytkowników tu było. szkoła o profilu...

Witrynaopen reading frames (ORF), orfH79 is a mitochondria chimeric gene being responsible for the CMS trait in Honglian (HL) rice. Weakly expressed ORFH79 strongly inhibits … scotch map single maltWitryna7 maj 2013 · Recent results from our group suggest that ORFH79 is expressed throughout entire tissues and might be involved in the assembly of Complex V (Liu et … scotchmarkWitryna小木虫,论坛,科研. （1）类风湿因子人血清中IgM、IgG型类风湿因子（RF）可以与...在用多抗测定抗原时对测定结果影响不大，但在用单克隆抗体测定抗原时，假如交叉抗原决定簇正好是所用单克隆抗体相对应的靶决定簇... scotchmark 3921 38x10WitrynaorfH79) and CMS cytotoxic protein (ORFH79) accumulate when RFC3 is silenced in restorer lines. We presented the analysis with blue-native polyacrylamide gel electrophoresis, indicating that RFC is disrupted in the RNAi line. We concluded that RCF3 is indispensable as a scaffold protein for the assembly of the RFC complex. pregnancy breast tenderness vs periodWitrynamolecular mechanisms. Previously, orfH79 was cloned and identiﬁed as the CMS gene in Honglian rice, but how the ORFH79 protein causes pollen abortion is still unknown. Through bacterial two-hybrid library screening, P61, a subunit of the mitochondrial electron transport chain (ETC) complex III, was selected as a candidate that interacts … pregnancy breast pain second trimesterWitrynaWe propose that the CMS protein, ORFH79, can bind to complex III and decrease its enzyme activity through interaction with P61. This defect results in energy production … scotch marine boiler definitionWitryna本发明公开了一种DNA分子、一种重组载体、一种细胞和一种植物。本发明还公开了一种有效防止转基因植物花粉逃逸的方法，包括如下步骤：步骤一、将DNA分子构建到表达载体上得到带有DNA分子的重组载体；步骤二、将重组载体转化到植物细胞中，筛选、培养得到含有DNA分子的转基因植株。本发明 ... scotchmark 7883